Identifikasi Kandungan Minyak Atsiri dalam Infusa Daun Ketumbar (Coriandrum sativum L.) sebagai Proteksi Ulkus Duodenum

Abstract

Duodenal ulcer disease is erosion or erosion and rupture of the mucosa due to a number of factors when the balance of gastric acid and pepsin secretion will affect the proportion of bicarbonate and prostaglandins, the most important as mucosal defense from acid and pepsin. Aspirin is a class of non-steroidal anti-inflammatory drugs which has a mechanism of action, namely inhibiting cyclooxygenase (COX) enzymes, especially cyclooxygenase-1 (COX-1), resulting in inhibition of prostaglandin and thromboxane biosynthesis from arachidonic acid. This study aims to examine the ability of infusion of coriander leaves (Coriandrum sativum L.) as protection of the duodenal mucosa in aspirin-induced rats. Samples of fresh coriander leaves (Coriandrum sativum L.) were infused using water as a solvent. Phytochemical characterization and screening of IDK was carried out and testing using GCMS with the results of the analysis showing that there were 36 peaks with different retention times. Tests were carried out on male rats, which consisted of 6 groups. Group 1 as a normal control was given drinking water without an inductor, group 2 as a negative control was given drinking water + 800 mg aspirin, group 3 as a positive control was given sucralfate suspension 360 mg/kg, group 4 was given coriander leaf infusion at a dose of 20%, group 5 was given 40% dose of coriander leaf infusion, and group 6 was given 80% dose of coriander leaf infusion. The ulcer protection test was carried out for 14 days, rats were given a negative control, positive control, infusion of coriander leaves and also an inductor at intervals of 2 days during the test. On the 14th day, the rats to be sacrificed were first fasted for 24 hours while still being given a drink, then anesthetized with 10 ml of chloroform and sacrificed by cervical dislocation. Dissected, then part of the duodenum was taken and observed macroscopically and microscopically for the histopathology of experimental animals. The results of statistical analysis showed that the normal group had a difference with the negative group, the 20% IDK group, the 40% IDK group and the 80% IDK group (p <0.05) but had no difference with the positive group with sucralfate suspension (p >0.05 Histopathological picture showed the occurrence of the duodenal mucosa of rats given sucralfate, IDK 20%, IDK 40%, and IDK 80% showed that the cohesion between mucosal cells was good and there was still erosion of the epithelial cells although not as big as the control administration negative. Based on the description, it can be concluded that IDK can protect the duodenal mucosa and improve cohesion between cells of the duodenal mucosa of ulcerated rats.