Analisis Metamfetamin pada Maggot Serangga Necrophagous Menggunakan Gas Chromatography- Mass Spectrometry (GCMS) sebagai Aplikasi Entomotoksikologi Forensik

Abstract

Cases of death of methamphetamine abusers in Indonesia were increasing, where bodies are generally found after several days of investigation, so forensic entomotoxicology uses insects to detect methamphetamine. The use of insects was chosen because insects are the first living things to infest corpses. This study aims to analyze methamphetamine found in necrophagous insect maggot grown on beef and pork using GCMS as an application of forensic entomotoxicology and to determine the validation method of the GCMS used. The methamphetamine contained in maggot was extracted by maceration and sonication methods using methanol:chloroform 1:3 and then a preliminary test was carried out using the Marquis reagent which changed the color of the sample to brownish orange. In the qualitative test, methamphetamine was obtained from day 1 to 8 for in respectively : 26.00, 42.50, 30.53, 49.57, 47.78 μg/mL and in pork respectively: 16.95 , 30.92, 27.94, 434.55, and 34.23 μg/mL. The TLC test produced an Rf value close to the comparator Rf value (0.648) which indicated that maggot was positive for methamphetamine. The validation of the GCMS method for methamphetamine compounds shows an accuracy value with a percent recovery (% recovery) of 101,896 % with a straight line equation y = 190269.56x – 970.296 with a correlation value (R) of 0.991. The precision test value with a standard deviation of 0.420 and a Relative Standard Deviation (RSD) of 1,186 % is <20%. The LOD and LOQ values were 6.6222 x 10-6 μg/mL and 1.2514 x 10-4 μg/mL respectively.MS.