Hubungan Polimorfisme Genetik CYP2A6 dengan Metabolisme Nikotin pada Laki-Laki Perokok di Kota Medan

Abstract

BACKGROUND: Nicotine is one of the main substances in cigarettes which plays an important role in the level of dependence on smoking in a person. The presence of nicotine in the urine of a smoker can indicate the metabolic activity of nicotine in the body of a smoker. CYP2A6 is an important enzyme that plays a role in the metabolism of nicotine to cotinine and cotinine to trans-3-hydroxycotinine. AIM: This study aims to analyze the correlation between nicotine metabolism and genetic polymorphism CYP2A6 in male smokers in Indonesia METHODS: This study used a cross sectional study method with a consecutive sampling technique of 100 male smokers aged between 20 to 65 years who met the inclusion and exclusion criteria. CYP2A6 genetic polymorphism was examined by PCR Restriction Fragment Length Polymorphism (RFLP) through venous blood extraction. The level of nicotine metabolites in urine samples was assessed by High Performance Liquid Chromatography (HPLC) examination. The relationship between nicotine metabolism and genetic polymorphisms of the CYP2A6 gene in male smokers in Indonesia was determined by logistic regression using Epi Info-7 software. RESULTS: There were 100 participants involved in this study, which 78 subjects were with the first stage of CYP2A6 polymorphism. There was no significant correlation between CYP2A6 genotype and nicotine metabolism (p value > 0.05). The 1B/1B genotype and *1A allele were more frequently found in the population in this study. Most research subjects had rapid metabolizers level. CONCLUSION: There is no significant relationship between genetic polymorphisms of CYP2A6 and nicotine metabolism in male smokers in Indonesia, so it is important to conduct further studies to investigate the relationship between genetic polymorphisms and nicotine metabolism in different populations.