| dc.description.abstract | Background: The use of Doxorubicin (DXR) in the treatment of cancer is
increasing, with increasing cancer morbidity. The nephrotoxic effect of DXR is
still a problem. Puguntano leaf ethanol extract as an anti-inflammatory can be
nephroprotective. So the combination of DXR and ethanol extract of Puguntano
leaves will reduce the nephrotoxic effect of DXR.
Objective: This study aims to explain the differences in the expression of TGF-
β1, E-selectin, glomerulosclerosis, interstitial fibrosis, urea, creatinine, and
proteinuria under normal, nephrotoxic and nephroprotective conditions.
Methods: The design of this study was an experimental study, with a Posttest
with Control Group. This research was conducted at the USU Medical Faculty
Research Unit Laboratory, USU Medical Faculty Research Unit Laboratory of
Anatomical Pathology, USU Medical Faculty Research Unit Integrated
Laboratory and USU Hospital Laboratory. Thirty male wistar mice were divided
into three groups, namely the control group without treatment (K1), the treatment
group (K2) injected with DXR, and the therapeutic group (K3) injected with DXR
intraperitoneally (ip) and ethanol extract of Puguntano leaves orally galvage, each
group 10 tails. The injection was done once a week for three consecutive weeks
and the ethanol extract of Puguntano leaves orally galvage with a dose of 200
mg/kg body weight per day for three weeks. In the 4th post-treatment week, each
group was executed by administering inj. Ketamine 75 mg/kg body weight
intraperitoneally. Examination of TGF-β1 and E-selectin expression by
immunohistochemical technique using monoclonal antibody. Histopathological
examination of glomerulosclerosis and interstitial fibrosis, using Masson's
Trichome. Routine blood tests, urea and creatinine using immunoturbidimetry
technique urine dipstick. Data analysis using ANOVA test.
Results: The results showed that DXR increased (p value = 0.001) TGF-β1
expression (253.5%±65.1), E-selectin (218.0±70.4), glomerulosclerosis,
interstitial fibrosis, proteinuria, urea and creatinine. On the other hand, the
administration of Puguntano leaf ethanol extract combined with DXR caused a
decrease (p value = 0.001) TGF-β1 expression (180.0 ± 51.0), E-selectin (172.5 ±
57.98), glomerulosclerosis, interstitial fibrosis, proteinuria, urea, and creatinine.
Conclusion: The combination of DXR and ethanol extract of Puguntano leaves
can be used to reduce the nephrotoxic effect of DXR. | en_US |
