Aktivitas Antibakteri Ekstrak Etanol Herba Poguntano (Picria fel-terrae Lour.) terhadap Staphylococcus epidermidis dan Propionibacterium acnes

Abstract

Background: Poguntano herb (Picria fel-terrae Lour.) is one of the species that often used by people in Berastagi, Karo, North Sumatra as medicine for various diseases. Poguntano herb contains of alkaloids, flavonoids, glycosides, saponins, steroids/triterpenoids, and tannins which have the potential as antibacterial. Objective: The purpose of this research was to determine the antibacterial activity of ethanol extract of poguntano herb (Picria fel-terrae Lour.) against Staphylococcus epidermidis and Propionibacterium acnes. Methods: This research included the manufacture of dried powder, characterization of dried powder, phytochemical screening, extraction by maceration method as well as testing of antibacterial activity against Staphylococcus epidermidis and Propionibacterium acnes with agar diffusion method using paper discs. Results: The results indicated that the dried powder of poguntano herb (Picria fel terrae Lour.) have water content of 7.9979%; water soluble extract content of 22.0498%; ethanol soluble extract content of 14.9367%; total ash content of 5.4317% and acid insoluble ash content 1.3248%. The ethanol extract of poguntano herb contained alkaloids, steroids/triterpenoids, flavonoids, glycosides, tannins, and saponins. Antibacterial activity test showed that ethanol extract of poguntano herb inhibited the growth of Propionibacterium acnes at the MIC 1.56 mg/ml with diameter of 8.13±0.05 mm and Staphylococcus epidermidis at the MIC 1.56 mg/ml with diameter of 7.06±0.05 mm. Conclusion: The ethanol extract of poguntano herb (Picria fel-terrae Lour.) has antibacterial activity against Staphylococcus epidermidis and Propionibacterium acnes.