Efikasi Antibakterial Larutan Irigasi Sodium Hipoklorit dan Kitosan Oligosakarida terhadap Enterococcus Faecalis pada Perawatan Endodontik dengan Instrumentasi yang Berbeda

Abstract

The main goal of root canal treatment is to clean and disinfect the root canal system by eliminating bacteria and removal of necrotic tissue. Mechanical instrumentation alone is not enough to clean the root canal, so it is necessary to use irrigating materials to reach the root canal anatomy that cannot be reached by preparation instruments This study aimed to determine the differences in root canal irrigation and instrumentation on the formation of E. faecalis bacterial biofilm and the formation of dentin residue. Twenty-four single canal mandibular premolars which had been extracted for orthodontic needs were divided into four groups. All groups underwent decoronation. Teeth were divided into four groups, namely A (sample irrigated with 2.5% NaOCl and 17% EDTA and prepared with F-One Blue), B (sample irrigated with 2.5% NaOCl and 2% Chitosan Oligosaccharide and prepared with F-One Blue ), C (sample irrigated with 2.5% NaOCl and 17% EDTA and prepared with One-Curve), D (sample irrigated with 2.5% NaOCl and 2% Chitosan Oligosaccharide and prepared with One-Curve). Then, an examination of E. faecalis biofilm and dentin residue was carried out. The data obtained was subjected to a One-way ANOVA statistical test and continued with the LSD Post Hoc test. The One-way ANOVA test results showed that there was a significant difference in the growth of E. faecalis biofilm between the treatment groups. From the research results it was found that Groups B and D had a very good ability to reduce the mass of E. faecalis biofilm Based on the scatter analysis, the group with root canal irrigation using 2.5% NaOCl and 2% COS and instrumentation with F-One Blue showed the ability to eliminate E. faecalis and inhibit the optimal formation of dentine residues.