Hubungan Paparan Asap Rokok terhadap Histopatologi Laring pada Tikus Rattus Novergicus

Abstract

Background: Cigarette smoking can cause health problems for both active and passive smokers because the toxic substances contained in it cause health problems and induced laryngeal histopathological changes. A study using mice (Leao et al, 2016) showed histopathological changes occurred in animals given passive exposure of cigarette smoke. The characteristic of chronic inflammation were found in the lamina propria of the larynx of the treated mice, characterized by the presence of mononuclear inflammatory cells, including lymphocytes, macrophages and eosinophils. Objective: To determine the relationship of cigarette smoke to the number of inflammatory cells (lymphocytes, macrophages and eosinophils), edema, hyperplasia, and dysplasia after exposure to cigarette smoke for 120 minutes in histopathology samples taken at 0, 10, 20, 30 and 40 days of observation. Methods: In this study used a true experimental design model with a post-test only group laboratory experimental design. A total of 25 mice were used in the experiment. Mice were divided into 5 groups of 5 animals each. Results:There was a change in the number of lymphocytes by 60% with p < 0.05 (p value = 0.043). There was no change in the number of macrophages, which averaged 80-100% with p > 0.05 (p value = 0.406) and eosinophils, which averaged 80% with p > 0.05 (p value = 0.126) and no hyperplasia changes as much as 40% with p > 0.05 (p value = 0.960). Conclusion: There is a statistical relationship to the number of lymphocytes. There is no relationship of macrophages, eosinophils caused by 120 minutes/day cigarette smoke exposure treatment for 0 days, 10 days, 20 days, 30 days and 40 days. There were no statistical relationship on edema, dysplasia, hyperplasia caused by 120 minutes/day cigarette smoke exposure treatment for 0 days, 10 days, 20 days, 30 days and 40 days.