| dc.description.abstract | Background: In traditional medicine, several euphorbiaceae plants such as the
candlenut plant, kamala plant, and firestorm plant are used to treat various
microbial diseases. However, the utilization of the leaves of this plant has not
been widely used. Candlenut leaves, kamala leaves and firestorm leaves are
known to contain flavonoids, tannins and saponins which can inhibit the growth
of bacteria.
Purpose: The purpose of this study was to determine the antibacterial activity of
the ethanol extract of candlenut leaves, kamala leaves, and firestorm leaves on the
growth of Pseudomonas aeruginosa.
Methods: This research included the manufacture of dried powder,
characterization of dried powder, phytochemical screening, extraction by
maceration method using ethanol 96% and testing the antibacterial activity of the
ethanol extracts of candlenut leaves, kamala leaves, and firestorm leaves
respectively diluted with DMSO into several concentration that is 500 mg/ml; 400
mg/ml ; 300 mg/ml ; 200 mg/ml ; 100 mg/ml ; 50 mg/ml ; 25 mg/ml ; 12.5 mg/ml
; 6.25 mg/ml ; 3.125 mg/ml with positive control of 0.1% Ciprofloxacin against
Pseudomonas aeruginosa using the paper disc diffusion method.
Results: Ethanol extracts of candlenut leaves, kamala leaves, and firestorm leaves
have antibacterial activity against Pseudomonas aeruginosa. The minimum
inhibitory concentration (MIC) of each extract is at a concentration of 6.25
mg/ml; 25 mg/ml; 12.5 mg/ml with a diameter of 6.66 ± 0.15 mm respectively;
6.36 ± 0.15mm; 6.5 ± 0.45mm.
Conclusion: Ethanol extracts of candlenut leaves, kamala leaves and firestorm
leaves have antibacterial activity | en_US |
