Uji Efek Imunostimulan Ekstrak VCO Kurkuminoid dari Rimpang Kunyit (Curcuma domestica Val.) pada Tikus yang Diinfeksi Staphylococcus aureus dan Diinduksi Siklofosfamid

Abstract

Turmeric (Curcuma domestica Val.) is one of plants growing in Indonesia that has numerous health advantages. Curcuminoid compounds are turmeric contents that are known to have immunomodulatory activity and have been proven in several in vivo test. Immunosupressive medication, such as cyclophosphamide, is widely used to treat cancer, which can cause susceptibility to infection. Therefore, the aim of this study is to determine the immunostimulant effect of curcuminoid VCO extract from turmeric rhizomes on white mice that have been infected with Staphylococcus aureus and induced by cyclophosphamide. Curcuminoid VCO extract is obtained using the Microwave Assisted Extraction (MAE) method with VCO as a solvent. Then quantitative analysis of the VCO curcuminoid extract is carried out using TLC-Densitometry. The experimental animals used are male mice which are given VCO curcuminoid extract at a dose of 1 mL, 0.5 mL, 0.25 mL, curcuminoid suspension 200 mg/kgBW 1 mL, levamisole suspension 25 mg/kgBW 1 mL, VCO 1 mL, and suspension CMC-Na 0.5% 1 mL as a negative control on days 1 to 14. On the 4th day, the experimental animals are infected with Staphylococcus aureus bacteria, then on days 4, 8, and 12 they are induced by cyclophosphamide 30 mg/kgBW, furthermore the immunostimulant effect is tested using the total leukocyte and differential leukocyte analysis method using a hematology analyzer, IgG and IgM antibody levels, levels of cytokines IL-2, IL-4, IFN- ℽ, and the amount of TLR4 using the ELISA method and microscopic examination of mice spleen pulp Hematoxylin Eosin preparations. The test results show that curcuminoid VCO extract 1 mL, 0.5 mL, 0.25 mL, curcuminoid suspension, levamisole suspension, and VCO are able to increase total leukocytes, leukocyte differential, antibody levels, cytokine levels, TLR4 levels, and decrease pulp diameter size of mice spleen with a significant difference (p < 0.05) to the CMC-Na 0,5% group as a negative control. Based on the results of this study, VCO curcuminoid extract can increase the number of total leukocyte and leukocyte differential, levels of cytokine IL-2, IL-4, and IFN-ℽ, TLR4 levels, and decrease spleen pulp diameter most effectively at a dose of 1 mL of curcuminoid VCO extract. Therefore, curcuminoid VCO extract has the potential to have an effect as an immunostimulant.