Pengaruh Penambahan Ekstrak Wortel dalam Pengencer Sitrat Kuning Telur Ayam Ras Terhadap Kualitas Spermatozoa Semen Beku Kerbau Sungai di UPTD Balai Inseminasi Buatan Provinsi Sumatera Utara

Abstract

This study aims to obtain the utilization of carrot extract in purebred chicken egg yolk citrate diluent on the quality of Sungai buffalo spermatozoa. This research was carried out the UPTD Department of Agriculture and Animal Husbandry of North Sumatera Province. The design used was a completely randomized design (CRD) with 5 Treatments and 4 replications. The treatment was given carrot extract consisting of P0 : 100% egg yolk citrate + 0% carrot extract; P1 : 77.5% egg yolk citrate + 22.5% carrot extract; P2 : 75% egg yolk citrate + 25% carrot extract; P3 : 72.5% + 27.5% carrot extract; P4 : 70% egg yolk citrate + 30% carrot extract. The observed parameters are motility, viability, abnormality, and mortality of spermatozoa. The results showed that the average spermatozoa motility were P0, P1, P2, P3, and P4, respectively, 12.5%, 16.25%, 8.75%, 7.5% and 4.5%. Spermatozoa viability were 21.75%, 24.75%, 19.25%, 17.75% and 13%, respectively. Spermatozoa abnormalities were respectively 22.5%, 20.87%, 21.87%, 23% and 25.25%. Spermatozoa mortality were 39.25%, 36.25%, 41.75%, 43.25% and 48%, respectively. The results of the analysis of variance showed that the treatment had a very significant effect (P<0.01) on the motility, viability, abnormality and mortality of spermatozoa. It can be concluded that the addition of 22.5% (P1) carrot extract in purebred chicken egg yolk citrate diluent can maintain the quality of river buffalo spermatozoa because it can provide the best results in maintaining the quality of river buffalo spermatozoa.