Uji Efektivitas Kombinasi Ekstrak Daun Singkong (Manihot Esculenta C.) dan Daun Pirdot (Saurauia Vulcani K.) sebagai Antibakteri terhadap Staphylococcus Aureus

Abstract

Introduction. One of the most common infectious diseases is caused by Staphylococcus aureus bacteria. Treatment is given in the form of antibiotics. However, irrational use of antibiotics can cause antibiotic resistance problems. For this reason, other alternatives are needed using herbal plants such as cassava leaves (Manihot esculenta C.) and pirdot leaves (Saurauia vulcani K.). Objective. To test the effectiveness of a combination of cassava leaf and pirdot leaf extracts as antibacterial against Staphylococcus aureus. Methods. This research is a laboratory experimental research using disc diffusion method and posttest only control group design. The antibacterial activity test was carried out using a combination of cassava leaves and pirdot leaves with a concentration ratio of 80%: 20%, 50%: 50%, 30%: 70%, positive control chloramphenicol 30 μg, and negative control DMSO 10%. Data analysis used Kruskall-Wallis non-parametric test. Results and Discussion. The results of measuring the inhibition zone of the combination of cassava leaf and pirdot leaf extracts against the growth of Staphylococcus aureus bacteria at concentrations of 80%: 20%, 50%: 50%, 30%: 70% produced inhibition zones of 14.14 mm, 26.47 mm, and 22.73 mm, respectively. The Kruskall-Wallis test results showed that the p-value of 0.00 indicates that the p-value is significant (<0.05), which means that there is a significant difference in the mean value of each treatment group. Conclusion. The combination of cassava leaf extract and pirdot leaf has antibacterial activity against Staphylococcus aureus bacteria and is more effective than its single administration. The best concentration of the combination of cassava leaf extract (Manihot esculenta C.) and pirdot leaf (Saurauia vulcani K.) that can inhibit the growth of Staphylococcus aureus bacteria is in the concentration ratio of 50%: 50% which produces an average inhibition zone diameter of 26.47 mm.