Isolasi dan Identifikasi Golongan Senyawa Flavonoida dari Buah Tumbuhan Butun (Barringtonia Asiatica (L.) Kurz)

Abstract

One of the plants that can be used for traditional medicine is butun (Barringtonia asiatica (L.) Kurz). The fruit can be used to control scabies, the leaves are commonly used to treat stomach aches and rheumatism and the seeds are used as a vermifuge. Several studies have also proven that butun fruit has good antibacterial, antifungal and antioxidant activity. One of the bioactive compounds that plays a role in this is flavonoids. Isolation of flavonoid compounds compounds from Butun Fruits (Barringtonia asiatica (L.) Kurz) has been carried out starting with the maceration extraction stage with methanol solvent. The methanol extract was concentrated and then dissolved in distilled water. The distilled water solution is extracted and partitioned with ethyl acetate, then the ethyl acetate layer is separated and concentrated. The concentrated extract of ethyl acetate was dissolved with methanol and partition extracted with n-hexane. The methanol layer is then evaporated until all the methanol has evaporated. The concentrated methanol extract was then analyzed by TLC, then separated by column chromatography with silica gel as the stationary phase and chloroform: ethyl acetate (60:40, 50:50, 40:60, 30:70, 20:80, and 10:90) v /v as the mobile phase. The third fraction was chosen to be purified by column chromatography with silica gel as the stationary phase and benzene: acetone (80:20) v/v as the mobile phase. The pure compound obtained was a yellow amorphous solid of 7 mg with a value of Rf = 0.41 using the eluent benzene: acetone (80:20) v/v. Next, the compounds obtained were analyzed using a UV-Visible Spectrophotometer, Infrared Spectrophotometer (FT-IR), and Proton Nuclear Magnetic Resonance Spectrophotometer (¹H-NMR). Spectroscopic data shows that the isolated compound allegedly is a flavonol group.