| dc.description.abstract | Isolation and identification of phenolic compound from cedar bay cherry leaves (Eugenia reinwardtiana DC.) have been carried out by maceration extraction using methanol as a solvent. The concentrated methanol extract was re-extracted using ethyl acetate repeatedly until it was negative for FeCl1 5%. The ethyl acetate extract was concentrated and redissolved with methanol and then partitioned with n hexane until the n-hexane layer was clear. The methanol extraxt was separated by column chromatography with a stationary phase of sihca gel and a mobile phase of chloroform: methanol (90:10; 80:20; 70:30; 60:40) vlv, respectively . The compound obtained from fraction 105-210 was pur(fied by preparative thin layer chromatography to produce 10 mg orange redness amorphous solid with an Rf value of0,40 with chloroform: methanol (80:20) v;v as the eluent. Analysis of the compounds obtained was carried out using UV-Vis spectrophotometry, FTIR, and 1H-NMR. The UV-Vis spectrum shows a wavelength (A max) of 208 nm in band II and 282 nm in band I. The FT-IR spectrum shows the presence of - OH stretching, CH stretching, C=O stretching, C=C alkene, C=C aromatic stretching, C-O stretching, respectively, at wave numbers 338, 1 21 cm·1, 2920,23 cm·1, 1720,50 cm·1, 1622,13 cm·1, 1516,05 cm· 1, 1463,97 cm·1, 1091,71 cm·1. Spectrum 1H-NMR shows the presence of protons H-2 and H-6 in the aromatic ring o,fphenolic compounds, proton from OCH1, proton o,f H- 7 and H-8. Based on the data obtained, the isolated compound obtained a phenolic compound which is suspected to be a compound in the phenylpropanoid group. | en_US |
