Isolasi dan Identifikasi Golongan Senyawa Flavonoida dari Buah Pacar Kuku (Lawsonia Inermis L.)

Abstract

Isolation and ident fication of flavonoid compounds from henna fruit (Lawsonia inermis L.) through phytochemical screening where the ethyl acetate extract of henna fruit is positiveforflavonoids with 5% FeCl3 reagent and Mg (s) + HCl (p), then extracted maceration with methanol solvent, then re-extracted with ethyl acetate, partitioned with n-hexane and obtained methanol concentrated extract. The methanol concentrated extract was separated by column chromatography with silica gel stationa1y phase and chloroform: ethyl acetate mobile phase. Fractions 150-185 were pur fied by preparative thin layer chromatography. The pure compound obtained is a yellow amorphous solid, has a mass of 12 mg and an Rf price of 0.72 with chloroform: ethyl acetate eluent 30:70 (vl v) and an Rf value of 0.78 with acetone: benzene eluent 40:60 (v/v). UV-Vis spectroscopic analysis with methanol solvent showed two absorptions with wavelengths (J..max) 348 and 267 nm. FT-IR spectroscopy showed the presence of OH, C-H sp3, C=O ketone, C=C aromatic, O-H bending, C-0 bending, and C-0 -C asymmetric and symmetric bending groups at wave numbers 3425.58; 2958.80; 2873.94; 1728.22; 1512.19; 1379.10; 1125.57; 1072.42; 1037.70 (cm-1), respectively. Proton core magnetic resonance spectroscopy (JH­ NMR) showed the presence o_fprotons H-8, H-6, H-2: H-5', H-6'. Spectroscopic data showed that the isolated compounds wereflavonol group.