Pengaruh Pemberian Susu Kefir dengan Penambahan Ekstrak Bonggol Nanas Terhadap Kadar Glukosa Darah Tikus Wistar yang Diinduksi Streptozotocin

Abstract

Preparation of kefir combination of goat milk and almond milk using pineapple fruit tuber extract 5%. The variation used in this study was the ratio of goat milk: almond milk (v/v) with a ratio of 20:80, 30:70, 40:60, and 50:50. The combination kefir obtained was tested for protein content using the kjeldahl method and antioxidant test using the free radical scavenging method with 2,2-diphenyl-1- picrylhydrazyl (DPPH) reagent. This study aims to validate the effect of probiotic kefir combination of goat milk and almond milk that has been added with pineapple stem extract on hyperglycemia in Wistar rats induced by (STZ). Validation was done by measuring blood sugar levels of blood samples of STZ-induced rats. Pretest - posttest control group study design was conducted on 7 hyperglycemia-induced male Wistar rats 60mg/kg bw STZ dissolved in citrate buffer with pH 4.5. Rats were randomly grouped into three groups, namely: (1) group of animals in STZ as a positive control, (2) group of animals in STZ and given a combination of kefir 0.09 mL /bb / 21 days, (3) group of animals in STZ and given a combination of kefir 0.018 mL /bb / 21 days. The optimum protein content of kefir was obtained in the variation of 40:60 which amounted to 1,4412%, and for the antioxidant suppression results in the variation obtained amounted to 132,7653 ppm. Validation results obtained a dose of 0.018 mL /bb as the best variation with 3 weeks as the optimum time to reduce blood sugar levels in STZ-induced rats.