| dc.description.abstract | Phosphorus (P) is a key nutrient for agricultural biology and productivity because it plays an important role in almost all major metabolic processes in plants such as photosynthesis, energy transfer, macromolecular biosynthetic signal transduction and respiration. The problem of P in the soil is its low availability, which can become an obstacle to plant growth and production. To increase P that is unavailable to plants can be done through inoculation of potential phosphate solubilizing microbes in the soil. Sound could effect on phosphate solubilizing microbes activity and plant growth, because sound wave has a wide signal in nature and interacts with living organisms. The purpose of this study was to prove the effect of sound on phosphate solubilizing microbes activity, growth and yield of maize.
This study was divided into three steps, stage I was conducted to obtaining phosphate solubilizing microbes isolated from the soil rhizosphere of maize (Zea mays L.) by applying sound effects. The research was conducted in the laboratory of Faculty of Agriculture UMSU, such as isolation, qualitative testing, identification and testing of microbial growth curves. The sound that was played is the murottal sound of the recitation of the Al-Qur'an Surah Ar Rahman with an amplitude of 25 Hz. Genetic identification using PCR-16S rRNA Sequencing method. The results showed two isolates of phosphate solubilizing bacteria with the highest solubilization index in the rhizosphere soil without sound treatment were Burkholderia contaminans and Burkholderia latent, while isolates of phosphate solubilizing bacteria with the highest solubilization index in the rhizosphere soil with sound treatment were Burkholderia cepacia and Burkholderia sp. Phosphate solubilizing fungi were only found in rhizosphere soil with sound treatment. two isolates of phosphate solubilizing bacteria with the highest solubilization index were Talaromyces muroii and Talaromyces sp. Furthermore, this phosphate solubilzing microbes were used in step II.
Step II was conducted to comparing the effects of murottal and mujawwad sounds of reading Al-Qur'an surah Ar Rahman on the activity of phosphate solubilizing bacteria B. contaminans and Burkholderia sp. and the phosphate solubilizing fungi Talaromyces sp. and also to determine the phosphate solubilizing bacteria with the best activity. The research was conduct in the laboratory of the Faculty of Agriculture UMSU to measure the growth curve and the phosphate solubilization index. Determination of organic citric, malic and acetic acids using the 18-5-32 / MU / SMM-SIG (UPLC) method. The research method used was a separate plot design (SPD) with the main plot, namely the sound (S), there are 3 levels: S0 = silent, S1 = murottal sound of reading the Qur'an, amplitude 25 Hz and S2 = sound of mujawwad reading Al Qur ' an, amplitude of 50 Hz. Subplots were the types of microbes (M) with 3 levels, M1 = B. contaminans, M2 = Burkholderia sp. and M3 = Talaromyces sp. The results showed that sound had an effect on the phosphate solubilizing microbes activity, such as increasing growth, phosphate solubilization index and acetic acid levels. After calculating with a Likert scale, the phosphate solubilizing bacteria with the best activity was Burkholderia sp. The sound that most influences the activity of the phosphate solubilizing microbes was the voice of mujawwad reciting the Al-Qur’an, amplitude of 50 Hz. Phosphate solubilizing microbes and selected sound were applied to maize in the field.
The step III was conducted to combine sound treatment technology and phosphate solubilizing microbes to increase the growth and yield of maize (Zea mays L.) in the field of Lau Bekeri Village, Kutalimbaru District, Deli Serdang Regency in Inceptisol soil that has andic. The research method used was a separate plot design (SPD) with 3 repetitions, consisting of the main plot, namely the sound (S) with S0 = no sound and S1 = the sound of mujawwad, amplitude 50 Hz, the sound played was the reading of Al Qu'ran surah Ar. Rahman on the sound level meter 85 ± 5 dB. The sub plot factor was phosphate solubilizing microbes (M) with different doses, namely: M0 = without phosphate solubilizing microbes, M1 = Burkholderia sp. (10 ml/plant), M2 = Burkholderia sp. (20 ml/plant), M3 = Burkholderia sp. (30 ml/plant), M4 = Talaromyces sp. fungi (10 ml/plant), M5 = Talaromyces sp. fungi (20 ml/plant), M6 = Talaromyces sp. fungi (30 ml/plant). The results showed that the application of mujawwad sound with amplitude of 50 Hz (S1) gave better maize growth for all measured parameters compared to without the application of sound (S0). The application of sound had a significant effect on the parameters of 100 seed shelled weight, available P in soil, total K in soil and plant P uptake. Inoculation of the phosphate solubilizing microbes Burkholderia sp. (M2) or Talaromyces sp. (M5) of 20 ml per plant increased the growth and yield of maize and had a significant effect on the observed parameters of 100 seeds of shelled weight and total N in soil. The combination of sound treatment and phosphate solubilizing microbes inoculation significantly interacted with the observed parameters of plant height, number of leaves and stem diameter at 45 days after planting.
It is proven that the application of sound can increase the growth and activity of indigenous phosphate solubilizing microbes, in the cultivation of maize plants in Lau Bakeri Village, Kutalimbaru District, Deli Serdang Regency, it can be suggested to apply the sound of mujawwad amplitude of 50 Hz together with the phosphate solubilizing microbe inoculation of Burkholderia sp. bacteria or Talaromyces sp. fungi as much as 20 ml/plant | en_US |
