Sintesis Isopropil Miristat dari Isopropil Alkohol dan Asam Miristat pada Perbandingan Rasio Molar dan Berat Enzim Lipase Menggunakan Radiasi Mikrowave

Abstract

The production of isopropyl myristate through the esterification reaction of myristic acid with, isopropyl alcohol has been carried oi t using the lipase en.zyme as a biocatalyst with microwave radiation. The JJarameters exa mined in this study were

the molar ratio o'f m yri stic acid to isopropyl alcohol with variatons of·1:1, 1:2, 1:3,

1:4, 1:5 and the ifl:fluence o_f the weight o_f the lipase enzyme with variations of 1%, 5% , 10%, 15%, 20%, 25%, 30%, 40%. T/1,e conversion of isopropyl niyristate obtained was determined by alkalimetric titration and confirmed by using an FT-IR SfJec tro photometer. The highest conversion of iso propyl myristate for the molar ratio parameter of myristic acid to isopropyl alcohol of· 94.57 + 0.24% was obtained at reaction conditions of 1:2, microwave power 20%, catalyst weight 20%, and reaction tinze 1 min,itte, while .for the weigh.t of enzyme lipase para,neter maxi,-num conversion of 94.68 + 1.33% was obtained under reaction conditions of 20% micro1>va ve power , 5% catalyst 1>vei ght, 1 mi,-zute reaction time, and 1:2 molar ratio. The.fo rm a ti on , of 1PM is characterized by an, absorption band in the area of 1692.21 cm-1, proving the existence of· stretching vibrations of the carbonyl group (C=O) from the ester and is sitpported by stretching vibrations of -C-0-C- ester at wave numbers 1300 - 1000 cm-1.