Isolasi dan Identifikasi Golongan Senyawa Fenolik dari Daun Ceri Pantai (Eugenia Reinwardtiana DC.)

Abstract

Isolation and identification of phenolic compound from cedar bay cherry leaves (Eugenia reinwardtiana DC.) have been carried out by maceration extraction using methanol as a solvent. The concentrated methanol extract was re-extracted using ethyl acetate repeatedly until it was negative for FeCl1 5%. The ethyl acetate extract was concentrated and redissolved with methanol and then partitioned with n­ hexane until the n-hexane layer was clear. The methanol extraxt was separated by column chromatography with a stationa1y phase of silica gel and a mobile phase of chloroform : methanol (90:10; 80:20; 70:30; 60:40) vlv, respectively. The compound obtained from .fraction 105-210 was purified by preparative thin layer chromatography to produce 10 mg orange redness amorphous solid with an f value of 0,40 with chloroform : methanol (80:20) vlv as the eluent. Analysis of the compounds obtained was carried out using UV-Vis spectrophotometry, FTIR, and 1H-NMR. The UV-Vis spectrum shows a wavelength (J max) of 208 nm in band II and 282 nm in band I. The FT-IR spectrum shows the presence of - OH stretching, CH stretching, C=O stretching, C=C alkene, C=C aromatic stretching, C-O stretching, respectively, at wave numbers 3381,21 cm-1, 2920,23 cm-1, 1720,50 cm-1, 1622 ,13 cm- 1, 1516,05 cm- 1, 1463,97 cm-1, 1091,71 cm-1. Spectrum IH-NMR shows the presence o_f protons H-2 and H-6 in the aromatic ring ofphenolic compounds, proton.from OCH1, proton o_f H- 7 and H-8. Based on the data obtained, the isolated compound obtained a phenolic compound which is suspected to be a compound in the phenylpropanoid group.