| dc.description.abstract | Isolation and identification of phenolic compound from cedar bay cherry leaves (Eugenia reinwardtiana DC.) have been carried out by maceration extraction using methanol as a solvent. The concentrated methanol extract was re-extracted using ethyl acetate repeatedly until it was negative for FeCl1 5%. The ethyl acetate extract was concentrated and redissolved with methanol and then partitioned with n hexane until the n-hexane layer was clear. The methanol extraxt was separated by column chromatography with a stationa1y phase of silica gel and a mobile phase of chloroform : methanol (90:10; 80:20; 70:30; 60:40) vlv, respectively. The compound obtained from .fraction 105-210 was purified by preparative thin layer chromatography to produce 10 mg orange redness amorphous solid with an f value of 0,40 with chloroform : methanol (80:20) vlv as the eluent. Analysis of the compounds obtained was carried out using UV-Vis spectrophotometry, FTIR, and 1H-NMR. The UV-Vis spectrum shows a wavelength (J max) of 208 nm in band II and 282 nm in band I. The FT-IR spectrum shows the presence of - OH stretching, CH stretching, C=O stretching, C=C alkene, C=C aromatic stretching, C-O stretching, respectively, at wave numbers 3381,21 cm-1, 2920,23 cm-1, 1720,50 cm-1, 1622 ,13 cm- 1, 1516,05 cm- 1, 1463,97 cm-1, 1091,71 cm-1. Spectrum IH-NMR shows the presence o_f protons H-2 and H-6 in the aromatic ring ofphenolic compounds, proton.from OCH1, proton o_f H- 7 and H-8. Based on the data obtained, the isolated compound obtained a phenolic compound which is suspected to be a compound in the phenylpropanoid group. | en_US |
