Perbandingan Hasil Pemeriksaan Metode Cytocentrifuge Cytology dan Cell Block pada Cairan Pleura yang Dikonfirmasi dengan Pemeriksaan Imunohistokimia Calretinin sebagai Penanda Mesothelial Cell

Abstract

Background: Pleural effusion is an abnormal accumulation of fluid in the pleural cavity, which occurs as a result of disturbance in the balance between production and reabsorption. Cytologycal examination is important as diagnosis, determining the stage, guiding futher treatment for malignancy. Effusion fluid is often processed using cytocentrifuge cytology or cell block techniuques which have their advanteges and disadvantages. Calretinin as mesothelial cell marker valuable for establishing a more definitive cytopatholgical diagnosis. Objective: To know comparison of examination results cytocentrifuge cytology and cell block methods on pleure fluid confirm with calretinin immunohistochemistry as mesothelial cell marker. Materials and methods: Cross-sectional analytic observational study of 49 samples cytologically diagnosed pleural fluid specimens. Examination method cytocentrifuge cytology and cell block followed calretinin immunohistochemistry. Data analysis was tested with Chi square test. Results: The result of pleural effusion examination were confirmed by calretinin immunohistochemistry, which showed positive mesothelial cell in 45 people (91,8%), while those who did not show or negative mesothelial cell were 4 people (8,2%).