Isolasi dan Identifikasi Golongan Senyawa Flavonoida dari Kulit Batang Kimerah (Flueggea Virosa (Roxb. Ex Willd.) Royle)

Abstract

Isolation and identification of flavonoid compound from stem bark of kimerah (Flueggea virosa (Roxb. ex Willd.) Royle) have been carried out by maceration extraction using methanol as a solvent. The concentrated methanol extract was re-extracted using ethyl acetate. The ethyl acetate extract was concentrated and redissolved with methanol and then partitioned with n-hexane. The concentrated methanol extract was separated by column chromatography with a stationary phase of silica gel and a mobile phase of chloroform : methanol (90:10; 80:20; 70:30; 60:40) v/v. respectively. The compound obtained from fraction 105-190 was purified by preparative thin layer chromatography and produced 5.3 mg brownish yellow amorphous solid with an Rf value of 0.44 with chloroform : methanol (80:20) v/v. The compound obtained was analyzed using UV-Visible Spectrophotometer, Infrared Spectrophotometer (FT-IR), and Proton Nuclear Magnetic Resonance Spectrophotometer (¹H-NMR). Spectroscopic data shows that the isolated compound allegedly is a flavanone group.