Sintesis Isopropil Miristat dari Isopropil Alkohol dan Asam Miristat pada Perbandingan Rasio Molar dan Berat Enzim Lipase Menggunakan Radiasi Mikrowave

Abstract

The production of isopropyl myristate througli the esterification reaction of myristic acid with, iso prop)1l alcohol has been carried oi t using the lipase en.zyme as a biocatalyst with microwave radiation. The parameters examined in this study were

the molar ratio o'f m yristic acid to isopropyl alcohol with variatons of·1:1, 1:2, 1:3,

1:4, 1:5 and the i'7:fluence of the weight o,f the lipase enzyme with variations of 1%, 5% , 10%, 15%, 20%, 25%, 30%, 40%. The conversion of isopropyl niyristate obtained was determined by alkalimetric titration and confirmed by using an FT-IR SfJectro ph.otometer. The h.ighest conversion of iso propyl myristate for the molar ratio parameter of myristic acid to isopropyl alcohol of· 94.57 + 0.24% was obtained at reaction conditions of 1:2, microwave power 20%, catalyst weight 20 %, and reaction tinze 1 minitte, while for the l-vei gh.t of enzyme lipase para,neter maxitnum conversiori of 94.68 + 1.33% was obtained under reaction conditions of 20% microl-va ve power , 5% ca talyst weight , 1 mitzute reaction ti,ne, and 1:2 molar ratio. The.forma tion , of /PM is characte rized by an, absorption band in the area of 1692.21 cm-1, proving the existence of· stretching vibrations of the carbonyl group (C=O) from the ester and is supported by stretcliing vibrations of -C-0 -C- ester at wave numbers 1300 - 1000 cm-1.