| dc.description.abstract | Introduction. Typhoid fever is a digestive tract infection caused by Salmonella
typhimurium. The treatment given is antibiotics. However, irrational use of antibiotics can
cause resistance. So another alternative is needed by using herbal plants such as pirdot
leaf extract (Saurauia vulcani K.) and lime peel (Citrus aurantifolia). Objective. Testing
the effectiveness of a combination of pirdot leaf extract and lime peel as an antibacterial
against Salmonella typhimurium. Method. This research is a laboratory experimental
research using disc diffusion (in vitro) and a posttest only control group design.
Antibacterial activity tests were carried out using a combination of pirdot leaves and lime
peel with concentration ratios of 0%:100%, 25%:75%, 50%:50%, 75%:25%, 100%:0%,
positive control ciprofloxacin 5 μg and negative control distilled water. Data analysis used
the Kruskall-Wallis non-parametric statistical test. Results and Discussion. The results of
measuring the inhibitory zone of the combination of pirdot leaf extract and lime peel on
the growth of Salmonella typhimurium show that the concentration ratios of 0%:100%,
25%:75%, 50%:50%, 75%:25% produce inhibitory zones respectively. also 11.5 mm,
10.01 mm, 8.75 mm, and 6.85 mm. In a ratio of 100%:0% it is not effective because pirdot
leaves are unable to provide an inhibition zone for Salmonella typhimurium bacteria in
vitro. The positive control using ciprofloxacin produced an average inhibition zone of
41.02 mm, and the negative control using sterile distilled water did not produce an
inhibition zone. The results of the Kruskall-Wallis test showed a significant p-value of 0.00,
indicating a significant p-value (<0.05), which means there is a significant difference in
the average value of each treatment group. Conclusion. The combination of pirdot leaf
extract and lime peel has antibacterial activity and its effectiveness is demonstrated by lime
peel extract. | en_US |
