Efek Kombinasi Ekstrak Pegagan dan Ikan Gabus terhadap Kadar MDA Serum, Ekpresi Gen VEGF pada Jaringan Otak dan Fungsi Memori Spasial pada Tikus yang Diinduksi D-Galaktosa

Abstract

Background: During the aging process, the body's ability to cope with the effects of free radicals such as reactive oxygen species decreases. The process of membrane lipid oxidation due to free radicals produces lipid peroxidation products such as malondia ldehyde (MDA) which is often used as a marker of oxidative stress processes. One condition that is often found in the aging process is a decrease in cognitive function as a result of reduced endothelial angiogenic capacity and changes in microcirculation i n the brain. Increasing age causes a decrease in VEGF which is associated with reduced angiogenesis. Centella asiatica is a wild plant that also grows a lot in Indonesia which contains triterpenoids, madecassoside and asiaticoside which can inhibit reactiv e oxygen species production and revitalize blood vessels. Arginine can suppress oxidative stress and induce endogenous antioxidant responses. Objective: to determine the effect of giving combinations of centella asiatica and channa striatus extracts on MDA levels, VEGF gene expression in brain tissue and spatial memory function in D Galactose indunced in the rats. Methods: This study used a true experimental research using experimental animals using a post test only control group design research. Data analysis with the data normality test using the Shapiro Wilk test. For normally distributed data, an ANOVA test will be performed, followed by a multiple comparison analysis (post hoc comparison). For data that is not normally distributed, the Kruskal Wallis test will be carried out and will be continued with a double comparison, namely the Mann Whitney test. And for ca lculating VEGF gene expression using the Livak formula. Results: there was a significant difference in average serum MDA levels (p < 0.05). The lowest average serum MDA level was found in the combination group of centella asiatica and channa striatus extract (Channa Striata) 600/300 mg/kg BW (2.93 ± 0.39) and t he highest mean serum MDA level was found in the negative control group given injection of D Galactose 150 mg/kgBB (6.41±0.48). There were differences in gene expression between the treatment group an d the control group. There was a significant difference in latency time in Howell's Post Hoc Games test between treatment groups (p<0.05). Conclusion: MDA levels in the group given the combination of centella asiatica and channa striatus extract decreased significantly compared to the MDA levels in the control group and the group that was only given D galactose. After being treated for 6 week s, There was an increase in VEGF gene expression in the treatment and control groups. The combination of centella asi atica and channa striatus extract significantly reduced the latency time compared to the control group and the group that was only given D Galactose.