Perbandingan Daya Hambat Ekstrak Etanol Kulit Batang dengan Daun pada Mangga Arum Manis (Mangifera Indica L. Var Arum Manis) terhadap Pertumbuhan Staphylococcus Aureus

Abstract

Background. Infectious diseases are a growing problem. Cases of Staphylococcus aureus over the past 20 years have increased antibiotic resistance, opening up the potential for bioactive substances from natural ingredients to be studied, for example mango plants (Mangifera indica L.). All parts of this plant were studied to contain antibacterial substances. Objective. To determine the difference in inhibitory power between ethanol extract of stem bark and leaves of Mangifera indica L. var arum manis on the growth of Staphylococcus aureus. Method. True experimental design with Post Test Only Control Group Design. The treatment group was given stem bark extract and leaf extract of Arum Manis mango (Mangifera indica L. var. arum manis) each consisting of concentrations of 50%, 75% and 100%, with negative control sterile distilled water and positive control antibiotics. The measurement of antimicrobial activity by disc diffusion was seen from the diameter of the inhibition zone formed, measured using a caliper. Results. Inhibition zones were visible in all extract treatment groups. The highest inhibitory zone in bark extract was 100% concentration of 13.925 mm and the lowest in leaf extract of 50% concentration of 5.3 mm. The ANOVA test result found a p-value of <0.05. The results of the Independent-Sample T test showed a value of p=0.072. Conclusion. The phytochemical test results of arum manis mango leaf extract contain alkaloid compounds, flavonoids, glycosides, saponins, tannins and triterpenes / steroids. While arum manis mango bark extract contains alkaloids, flavonoids, glycosides, saponins and tannins. There was no significant difference between giving arum manis mango bark extract compared to arum manis mango leaf extract against the growth of Staphylococcus aureus ATCC 25923. The inhibitory power produced by the ethanol extract of arum manis mango bark is greater than the ethanol extract of arum manis mango leaves.