Efek Pemberian Salep Ekstrak Rimpang Kencur (Kaempferia Galanga L) terhadap Kadar Serum Interleukin-1β pada Tikus Putih (Rattus Norvegicus Strain Wistar) Model Laserasi Palpebra Partial Thickness

Abstract

Background: Ocular trauma in the form of lacerations on the eyelid or palpebral region is the most commonly encountered clinical finding. The use of medicinal plants such as the rhizome of Kaempferia galanga (KG), containing significant bioactive components like ethyl-p-methoxycinnamate (EPMC), as a therapeutic modality to enhance wound healing through its contained anti-inflammatory effects, has become a crucial focus in modern medicine. This study aimed to evaluate the influence of applying an extract ointment of KG on an experimental animal model of eyelid lacerations concerning the levels of interleukin-1β (IL-1β) in the serum, theoretically associated with the inflammatory process. Methods: This analytical experimental research employed a randomized post-test only control group design involving a cohort of Wistar strain white rats (Rattus norvegicus). Administration of the KG extract ointment at concentrations of 5%, 10%, and 15% was performed on a partial thickness eyelid laceration model, then compared with positive control (gentamicin ointment), negative control, and no intervention (sham) groups. Evaluation was conducted on serum IL-1β levels after a 5-day treatment period. Results: No statistically significant differences (P > 0.05) were found in each test group (n=5) following the interventions over 5 days. The lowest IL-1β levels were observed in the 5% KG group (10.23 ± 2.44 ng/ml), while the highest was in the 10% KG group (13.48 ± 2.27 ng/ml). The one-way ANOVA test conducted also concluded that no groups significantly differed from the sham population (11.09 ± 4.35 ng/ml). Conclusion: Administration of the KG extract ointment at any concentration did not affect the serum IL-1β levels in the experimental model of eyelid lacerations in animals.