Deteksi Gen Resistensi mecA dan mecC pada Methicillin Resistant Staphylococcus aureus (MRSA) dari Sampel Klinis RSUP H Adam Malik Medan

Abstract

Introduction. Staphylococcus aureus can cause infections in humans. Uncontrolled use of antibiotics causes Staphylococcus aureus strains to emerge that are resistant to beta-lactam antibiotics, known as Methicillin-resistant Staphylococcus aureus (MRSA). This resistance is caused by the presence of the mec gene, which causes a change in the Penicillin Binding Protein (PBP) to PBP2a, which causes the bacteria to have a low affinity for b-lactams. The mec resistance genes currently detected are the mecA, mecB, mecC, and mecD genes. Objective. This study aims to detect the presence and prevalence of mecA and mecC resistance genes in MRSA from clinical samples at H. Adam Malik General Hospital. Method. Clinical samples of infection were collected and identified using the VITEK-2 compact device. The detection of resistance genes is carried out using the Polymerase Chain Reaction (PCR) method. Visualization of the presence of genes is carried out using electrophoresis. Results. In this research, out of the 80 samples, 79 isolates (98.75%) were positive for the mec gene. The mecA resistance gene was found in 64 samples (80%), while the mecC gene was found in 78 samples (97.5%). A single mecA gene was found in one sample (1.25%), while a single mecC gene was found in 15 samples (18.75%). Conclusion. This study shows the presence of the mecC resistance gene in MRSA in humans. Isolates with the mecC gene were more numerous than the mecA resistance gene in MRSA H. Adam Malik General Hospital. These findings provide new information regarding the genetic profile of MRSA in Indonesia.