Pengaruh Pemberian Ekstrak Etanol Daun Binahong terhadap Penurunan Kadar Gula Darah pada Tikus Model Diabetes Melitus Tipe 2

Abstract

Background. Type 2 diabetes mellitus (T2DM) characterized by elevated levels of blood glucose. DM treatment using conventional medicine often causes side effects, so people are starting to switch to herbal medicine, such as Anredera cordifolia leaves or known as binahong. Binahong leaves contain various metabolites such as flavonoid which are known to have antidiabetic effects. Objective. To determine the effect of administering ethanol extract of Anredera cordifolia (Ten.) Steenis leaves on reducing blood sugar levels in T2DM rats. Methods. This study is a true experimental in vivo with pre-post test control group design. This study used 30 rats consisting of 6 groups: normal control, negative control, positive control (metformin), ethanol extract of binahong leaves dose 25 mg/kgBW, ethanol extract of binahong leaves dose 50 mg/kgBW and ethanol extract of binahong leaves dose 100 mg/kgBW. The treatment group was induced by HFD for 14 days and injected with streptozotocin 30 mg/kgBW followed by ethanol extract of binahong leaves for 14 days. Rat blood sugar levels were evaluated 7 and 14 days after administration of binahong leaf ethanol extract. Data analysis used parametric and Wilcoxon non-parametric statistical test. Results. Ethanol extract of binahong leaves dose 25 mg/kgBB, 50 mg/kgBB and 100 mg/kgBB had an effect on reduced blood glucose significantly. Conclusion. Ethanol extract of binahong leaves can reduce the blood glucose in rats significantly.