Pengaruh Pemberian Gel SH-MSCS terhadap Ekspresi IFN-γ (Studi in Vivo pada Tikus Model Diabetic Type 1 Foot Ulcer)

Abstract

Introduction. Diabetic foot ulcers is one of the most common complications in diabetes mellitus patients. Inflammatory cytokines play an important role in the course of diabetes and its complications. Stem cells-based therapies are increasingly being introduced into therapeutic approaches because they can repair tissues with their natural ability to produce cytokines, chemokines, and growth factors necessary to promote angiogenesis and extracellular matrix remodeling for healing. Objective. To prove the effect of SH-MSCs based gel on IFN-γ expression in diabetic type 1 foot ulcer model rats. Method. In vivo experimental study with a Post Test Only Control Group Design. The total sample was 24 male Wistar rats, divided into four groups. Group of healthy rats (SH) , group of diabetic type 1 foot ulcer model rats treated with based gel (K), group of diabetic type 1 foot ulcer model rats treated with SH-MSCs based gel with dose of 100 μL/kg weight in 100 mg gel (P1), and group diabetic type 1 foot ulcer model rats treated with SH-MSCs based gel with dose of 200 μL/kg weight in 200 mg gel (P2) and treated for 10 days. The levels of IFN-γ gene expression were tested using the non-parametric test Kruskal Wallis followed by the Mann-Whitney test. Results and Discussion. The result showed that the expression of IFN-γ a significant decrease between groups as indicated by the results of the Mann-Whitney test. Group K compared to P1 (p.0.004), group K compared to P2 (p.0.004), and group P1 compared to P2 (p.0.004). Conclusion. Administration of SH-MSCs-based gel had a significant effect on reducing IFN-γ expression in diabetic type 1 foot ulcer model rats.