Efek Pemberian Gel Ekstrak Daun Petai Cina (Leucaena Glauca, Benth) 6% dan 15% Terhadap Jumlah Fibroblas pada Penyembuhan Gingivitis Tikus Wistar Jantan

Abstract

Gingivitis is one of the most common types of periodontal disease in the world. This disease is caused by bacterial infection in plaque accumulation which releases endotoxins and causes inflammation in healthy gingival tissue. Fibroblast cells play an important role in the proliferation and maturation phases in accelerating the healing of gingivitis because of their ability to stimulate the formation of collagen fibers which will later repair damaged gingival connective tissue. The use of natural ingredients that have the potential to be developed in curing gingivitis, one of which is the administration of petai cina leaves. The content of flavonoids, saponins, tannins and alkaloids in petai cina leaves can have healing potential because it can trigger the proliferation of fibroblasts in the formation of new blood vessels and regeneration of damaged tissue. This study aimed to determine the number of fibroblast cells in induced rat gingiva Porphyromonas gingivalis ATCC33277 after administering 6% and 15% petai cina leaf extract gel. This research method used 40 samples of gingival tissue from the mandibular incisors of male Wistar rats (Rattus norvegicus) by following the inclusion and exclusion criteria. The research results are based on testsOne Way Anova showed that there was a significant difference in the number of fibroblasts (p<0.05) between treatment groups on day 5 and day 3. showed that no significant differences were found (p>0.05). Concentrations of 6% and 15% showed higher mean fibroblast values compared to the distilled water group and lower than the Gengigel group. This study concluded that 6% and 15% petai cina leaf extract gel had a significant effect on increasing the number of fibroblasts in the gingiva of mice experiencing gingivitis on the 5th day