Pengaruh Gel Ekstrak Daun Petai Cina (Leucaena Glauca, Benth) Terhadap Jumlah Neutrofil Tikus Wistar Jantan dalam Proses Penyembuhan Gingivitis

Abstract

Inflammation acts as a self-defense to phagocytize microorganisms that enter the body. Neutrophil cells are the first cells to migrate from blood vessels as a natural response of the body when damage signals occur. The aim is to determine the number of neutrophil cells in the gingiva of rats induced inflammation after the administration of Chinese petai leaf extract gel. The method used is true experiment with posttest only controlled group design. Male wistar rats were induced with Porphyromonas gingivalis ATCC®33277™ bacteria on the cervical gingiva of rats and waited for 4x24 hours until inflammation occurred in the rat gingiva. The gingiva of rats that experienced inflammation was treated in several groups, namely Chinese petai leaf extract gel concentrations of 6% and 15%, gengigel, and distilled water. On day 3 and day 5, dissection of each treatment group was carried out to see the number of neutrophil cells in rats using an Olympus CX31 microscope at 400x magnification. Data analysis used One Way ANOVA test then continued using the LSD Post Hoc test. The results showed that there was a decrease in the number of neutrophils in male Wistar rats that experienced gingivitis after being treated using Chinese petai leaf extract gel concentrations of 6% and 15% and there was no significant difference between Chinese petai leaf extract gel concentrations of 6% and 15%. The conclusion of this study is that Chinese petai leaf extract gel concentrations of 6% and 15% can reduce the number of neutrophils in rats that experience gingivitis