Isolasi dan Identifikasi Golongan Senyawa Fenolik dari Daun Tumbuhan Attarasa (Litsea cubeba (Lour.) Pers)

Abstract

The phenolic compound had been isolated from the leaves of Attarasa (Litsea Cubeba (Lour.) Pers). Isolation was carried out by maceration extraction method with methanol and methanol extact was re-extracted with ethyl acetate repeatedly until negative to 5% FeCl3. Ethyl acetate extract was concentrated and reconstituted with methanol and partitioned with n-hexane until the n-hexane layer was clear. The methanol extract was analyzed by thin layer chromatography and separated by column chromatography with a stationary phase of silica gel and a mobile phase of chloroform : methanol (90:10, 80:20, 70:30, 60:40) v/v . The compound obtained from fraction 50-100 was purified by preparative thin layer chromatography using chloroform : ethyl acetate ( 20:80) v/v eluent to produce a brown amorphous solid of 12 mg at Rf value of 0,82 using chloroform : methanol (80:20) v/v eluent. Based on the analysis of the UV-Visible spectrophotometer, there were two wavelength of λ1= 254 nm and λ2= 202 nm, the FT-IR spectrophotometer shows the presence of OH, C=O ester, C=C aromatic, C-H, and C-O grups successively at wave number 3427,51 cm-1, 2924,09 cm-1 ,1743,65 cm-1, 1463,97 cm1, 1377,17 cm-1, 1170,79 cm-1 .The proton Nucleus Magnetic Resonance Spectrophotometer (1H-NMR) shows the presence of doublet protons H-2, H-6 and H-3, H-5 on the benzene ring and methoxy protons, bins to the carbonyl group. Based on the data obtained by Spectrophotometer analysis, it was estimated that compound derived from phenolic acid.