| dc.description.abstract | The phenolic compound had been isolated from the leaves of Attarasa (Litsea Cubeba (Lour.) Pers). Isolation was carried out by maceration extraction method with methanol and methanol extact was re-extracted with ethyl acetate repeatedly until negative to 5% FeCl3. Ethyl acetate extract was concentrated and reconstituted with methanol and partitioned with n-hexane until the n-hexane layer was clear. The methanol extract was analyzed by thin layer chromatography and separated by column chromatography with a stationary phase of silica gel and a mobile phase of chloroform : methanol (90:10, 80:20, 70:30, 60:40) v/v . The compound obtained from fraction 50-100 was purified by preparative thin layer chromatography using chloroform : ethyl acetate ( 20:80) v/v eluent to produce a brown amorphous solid of 12 mg at Rf value of 0,82 using chloroform : methanol (80:20) v/v eluent. Based on the analysis of the UV-Visible spectrophotometer, there were two wavelength of λ1= 254 nm and λ2= 202 nm, the FT-IR spectrophotometer shows the presence of OH, C=O ester, C=C aromatic, C-H, and C-O grups successively at wave number 3427,51 cm-1, 2924,09 cm-1 ,1743,65 cm-1, 1463,97 cm1, 1377,17 cm-1, 1170,79 cm-1 .The proton Nucleus Magnetic Resonance Spectrophotometer (1H-NMR) shows the presence of doublet protons H-2, H-6 and H-3, H-5 on the benzene ring and methoxy protons, bins to the carbonyl group. Based on the data obtained by Spectrophotometer analysis, it was estimated that compound derived from phenolic acid. | en_US |
