Pengaruh Teknik Agitasi terhadap Kedalaman Penetrasi Ekstrak Lerak (Sapindus rarak DC) 12,5% sebagai Larutan Irigasi Ke dalam Tubulus Dentin di Sepertiga Apikal Saluran Akar: In Vitro

Abstract

Root canal cleaning and shaping is an important step in endodontic therapy. lerak extract is a natural irrigation solution. Bacteria can penetrate up to around 300-500 μm into the dentin tubules. These bacteria have the potential to cause Treatment failure, therefore, deep penetration of the irrigating agent into the dentinal tubules is highly desirable. The aim of this study was to determine the effect of sonic and ultrasonic agitation techniques on the penetration of irrigation solutions of 12.5% lerak extract, 3% NaOCl + 12.5% lerak extract and 3% NaOCl + 17% EDTA into the dentinal tubules in the apical third of the root canal. Thirty-six single-root mandibular premolars were decoronated at the Cementoenamel Junction (CEJ) level. Root canals were prepared with the S-One Blue #35.06 rotary file system. Teeth were divided into 6 groups, namely IA (12.5% lerak extract irrigation group + sonic agitation), IB (12.5% lerak extract irrigation group + ultrasonic agitation), IIA (3% NaOCL irrigation group + 12.5% lerak extract % + sonic agitation), IIB (NaOCl irrigation group 3% + lerak extract 12.5% + ultrasonic agitation, IIIA (irrigation group NaOCL 3% + EDTA 17% + sonic agitation), IIIB (irrigation group NaOCL 3% + EDTA 17 % + ultrasonic agitation). Dentin tubules were stained by irrigating the root canal with irrigation solution mixed with rhodamine b powder then agitated. All teeth were cut 4 mm from the apex, examined with CLSM (Confocal Laser Scanning Microscopy).The data obtained was subjected to a One-way ANOVA statistical test and continued with a Post Hoc test. The results of the One-way ANOVA test showed a significant difference (p<0.05) in the penetration of the 12.5% lerak extract irrigation solution into the dentin tubules in the apical third of the root canal compared to the other groups. However, the use of irrigation with sonic agitation (EDDY) and ultrasonic (Ultra-X) did not show a significant difference. From this research it can be concluded that the penetration of the 12.5% lerak extract irrigation solution combined with 3% NaOCl has the highest penetration into the dentin tubules when agitated with a sonic system. The use of agitation techniques using sonic or ultrasonic systems did not significantly differ in the penetration of the irrigation solution into the dentin tubules in the apical third of the root canal.