Uji Efek Hepatoprotektif Virgin Coconut Oil pada Tikus Wistar setelah di Induksi Isoniazid dan Rifampisin

Abstract

Impaired liver function is closely related to liver damage. The liver is prone to damage due to exposure to toxic materials. Rifampicin and isoniazid are first-line anti-tuberculosis drugs but are also the most potent hepatotoxic compounds. Virgin Coconut Oil (VCO) has antioxidant compounds that function to counteract free radicals. The antioxidants contained in Virgin Coconut Oil can function as hepatoprotective. This study aims to analyze the hepatoprotective effect of virgin coconut oil in rats induced by isoniazid and rifampicin. This study used 25 Rattus Norvegicus rats which were divided into 5 groups: normal controls were only given aquadest, positive controls were given Curcuma Force 3.6 mg/KgBW/day after 1 hour followed by isoniazid 100 mg/KgBW/day and rifampicin 100 mg /KgBW/day, negative control was given isoniazid 100 mg/KgBW/day and rifampicin 100 mg/KgBW/day, treatment group 1 was given VCO 1 ml/KgBW/day after 1 hour followed by isoniazid 100 mg/KgBW/day and rifampicin 100 mg/KgBW/day, and treatment 2 was given VCO 3 ml/KgBW/day after 1 hour, followed by isoniazid 100 mg/KgBW/day and rifampicin 100 mg/KgBW/day. The treatment was carried out every day for 14 days, then surgery and blood sampling were performed. Blood sample for examination of liver damage biomarkers (TB, ALT, AST) as well as the liver for macroscopic and microscopic examination. Data were analyzed using one way-ANOVA and then using Post Hoc Turkey HSD. The results showed that the changes in ALT, AST and total bilirubin values in the test animals showed statistically significant differences (p<0.05) compared to negative controls and normal controls as well as histopathological images of the liver in the form of fatty degeneration, hydropic degeneration, necrosis and congestion. Based on the results of the study it was concluded that VCO could act as a hepatoprotective. VCO at a dose of 1 ml/KgBB/day and VCO at a dose of 3 ml/KgBW/day can reduce ALT, AST and total bilirubin values and can reduce liver damage.