Uji Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM) Ekstrak Kulit Buah Nangka (Artocarpus heterophyllus) terhadap Pertumbuhan Bakteri Streptococcus mutans secara In Vitro

Abstract

Streptococcus mutans is a dominant bacterium found at the beginning of plaque formation until the formation of dental caries. Caries if not treated properly can lead to odontogenic infections. Caries prevention can be done by using antimicrobial agents. Antimicrobial materials from nature are currently widely used due to the decreasing effectiveness of chemical drugs due to side effects and resistance. Plants that have the potential to be used are jackfruit plants. On the skin of the jackfruit there is a sap/latex that is used by the community as a cure for abscesses. Jackfruit peel extract is known to contain antimicrobial active compounds, namely flavonoids, tannins and saponins. The purpose of this study was to determine the Minimum Inhibitory Concentration and Minimum Bactericidal Concentration of jackfruit peel extract on the growth of Streptococcus mutans. This type of research is a laboratory experiment with a post test only control group design. The concentration of jackfruit peel extract tested was 3,125% 6,25% ,12,5% 25%, and 50% with positive control (aloe vera mouthwash formula), and negative control (DMSO) four repetitions. Antibacterial effectiveness test using Kirby-baurer disc diffusion and agar dilution method. The results of data analysis using ANOVA showed a p-value of 0.00 on inhibition, which means jackfruit peel extract can inhibit the growth of Streptococcus mutans but Minimum Inhibitory Concentration and Minimum Bactericidal Concentration were not found. The conclusion of the study is that jackfruit peel extract can inhibit the growth of Streptococcus mutans bacteria but is not able to kill these bacterium.