Efektivitas Penggunaan Film Kitosan Cangkang Kepiting Hitam (Scylla serrata) terhadap Penyembuhan Luka Insisi Mukosa Rongga Mulut Tikus Galur Wistar

Abstract

A wound is the disruption of tissue continuity due to injury or surgery. Surgical procedures involve an incision or cutting process of tissues that result in wounds and tissue damage. Wounds are capable of healing on their own, but the healing process is relatively long and can cause discomfort. Prolonged wound healing may increase the potential for complications such as infection and inadequate blood supply, thereby inhibiting the healing process. Giant mud crab shell is a natural material containing chitin that can be processed into chitosan, which has the potential to aid in the healing of incision wounds in the oral mucosa. This study aims to determine the effectiveness of giant mud crab shell chitosan film on wound healing of incision wounds in the oral mucosa of Wistar rats. This research is a true experimental study with the sample of 25 Wistar rats divided into five groups, namely Aloclair group as a positive control, chitosan film 1%, 2%, 3%, and CMC-Na as the negative control. The research was conducted by observing erythema and length closure of wounds on days 1, 3, and 7. Based on the results of Pearson chi-square and Kruskall Wallis tests, it is shown that the use of chitosan film 1%, 2%, and 3% is effective in healing incision wounds in the oral mucosa of Wistar rats, with a concentration of 2% chitosan film being the most effective.