Ekspresi Imunohistokimia CD133 sebagai Penanda Sel Punca Kanker pada Karsinoma Sel Skuamosa Rongga Mulut Berdasarkan Klinikopatologi di RSUP Haji Adam Malik Medan

Abstract

Oral squamous cell carcinoma (OSCC) is a malignant neoplasm that is often found in the oral cavity. Most patients come to the dentist's practice with advanced cancer so that OSCC treatment is unsatisfactory. Apart from that, the presence of cancer stem cells is also a factor that needs to be considered. Cancer stem cells can be identified by conducting an immunohistochemical examination for CD133 antibodies. The aim of the study was to determine the expression of CD133, and the correlation to OSCC patients at Haji Adam Malik General Hospital Medan in 2019-2023 to age, gender, tumour location (clinical data) and histopathological sub-type/grading. The research method is descriptive analytical with a cross-sectional approach on 35 paraffin block samples diagnosed as OSCC obtained from H. Adam Malik General Hospital Medan in 2019-2023, and CD133 (HL1271, GeneTex, USA) immunohistochemical examination was done in the Pathology Anatomy Laboratory Medical Faculty of USU. Microscopically viewed under an Olympus CX21 light microscope. The results showed that CD133 appeared positive in 25 cases (71.4%) of the total 35 cases of OSCC. The Chi Square test showed a significant relationship between CD133 expression and the histopathological grading of OSCC. The conclusion of this study shows the role of the CD133 marker as a marker for cancer stem cells in oral squamous cell carcinoma.