Perbedaan Efektivitas Ekstrak Daun Asam Jawa (Tamarindus indica L.) dan Ekstrak Daun Manggis (Garcinia mangostana L.) terhadap Porphyromonas gingivalis ATCCÒ 33277Ô(In Vitro)

Abstract

Introduction Tamarind leaves (Tamarindus indica L) are used as traditional medicine and have bioactive components of saponins, flavonoids, streoids as antibacterial. Bioactive components of flavonoids, tannins, phenolics as antibacterial. The overall role of the phytochemicals of tamarind leaves and mangosteen leaves causes leakage of protein and enzyme functions from within the cell thus damaging membrane permeability causing bacterial lysis. Porphyromonas gingivalis often colonizes in the subgingival area causing gingivitis if not treated can cause periodontitis. Treatment is done with alternative herbs, namely tamarind leaves extract and mangosteen leaf extract against Porphyromonas gingivalis. Purpose of the study was determine the inhibitory power, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of tamarind leaf extract and mangosteen leaf extract. Method is experimental laboratory post test only control group design. Inhibition testing using blank disk diffusion method with digital calipers, determination MIC and MBC dilution method. Preparation of extract by maceration method from each concentration added to bacterial suspension, 4 repetitions and then observations were made. Parametric analysis using Oneway Anova Test and Post-hoc LSD test with a significance value of p<0.05, if the data obtained is not normally distributed and homogeneous, non parametric analysis with Kruskal-Wallis test and Mann Whitney test. The result of the study of the inhibition of tamarind leaf extract concentrations of 6,25%, 12,5%, 25%, and 50% diameter 15 ± 0.54 mm, 19.56 ± 0.12 mm, 20.93 ± 0.12 mm, and 23.37 ± 0.43, while the inhibition of mangosteen leaf extract concentrations of 6,25%, 12,5%, 25%, and 50% diameter 0, 10.43 ± 0.433 mm, 14.43 ± 0.31 mm, and 19.19 ± 0.555 mm. The effectiveness of tamarind leaf extract of several concentrations against Porphyromonas gingivalis ATCCÒ 33277Ô bacteria is MCI 6.25% and MBC 12,5%, while the effectiveness of mangosteen leaf extract MCI 6,25% and MBC 50%. Conclusion tamarind leaf extract is more effective than mangosteen leaf extract against Porphyromonas gingivalis ATCCÒ 33277Ô bacteria.