Pengaruh Edible Coating Selulosa Kulit Markisa Ungu (Passiflora edulis) pada Basis Gigi Tiruan Resin Akrilik Polimerisasi Panas terhadap Jumlah Candida albicans

Abstract

Polymethylmethacrylate (PMMA) is the most widely used base material in fabricating removable dentures, but has high porosity level which can cause the accumulation of biofilm, which one is Candida albicans. The attachment of Candida albicans on denture base can cause denture stomatitis. Edible coating on denture base can help to inhibit the rate of oxygen transfer thereby preventing aerobic microbial growth. Purple passion fruit peels contain high level of cellulose, which can be used as basic material in making edible coating. The purpose of this study is to examine the effect of cellulose edible coating from purple passion fruit peels on the number of Candida albicans on PMMA denture base. This laboratory experimental study used 40 PMMA samples measuring 10 x 10 x 1 mm, divided into 4 groups: a control group, groups with 1%, 2%, and 3% cellulose edible coating. Group samples with cellulose edible coating were dipped into the edible coating for 7 minutes. All samples were contaminated with 1 x 108 CFU/ml Candida albicans, cultured, and incubated for 48 hours at 37C and the number of colonies were counted. Data were analyzed using univariate and oneway ANOVA test. The results showed that 1%, 2%, and 3% cellulose edible coating from purple passion fruit peels could prevent the growth of Candida albicans on PMMA denture base (p<0.05). The most effective concentration in preventing the growth of Candida albicans on PMMA denture base is 1% cellulose edible coating from purple passion fruit peels.