Uji Antibakteri Obat Kumur Ekstrak Umbi Sarang Semut (Myrmecodia pendans) terhadap Staphylococcus aureus ATCC® 25923TM pada Kawat Stainless Steel

Abstract

Fixed orthodontic appliances users generally have difficulty in cleaning the oral cavity, causing plaque accumulation, bacteria increase, and thus higher risk of periodontal disease. The main bacteria in the plaque accumulation is Staphylococcus aureus. The use of antibacterial agent is one of the preventive measures to overcome this problem, hence the search for potential medicinal plants as antibacterial agent increasing as the use of natural ingredients have fewer side effects compared to the use of chemical alternatives. One of the medicinal plants that can be used as an alternative is ant-nest plant (Myrmecodia pendans). The aim of this research is to determine the effectivity of ants nest (Myrmecodia pendans) extract mouthwash on the amount of Stapyhlococcus aureus ATCC® 25923TM on stainless steel wires. The method of this research is an experimental laboratorium with post-test only control group design. The research started from making ant-nest plant (Myrmecodia pendans) mouthwash in concentration 0,8%, 1,6%, and 3,2%. Then, antibacterial testing was carried out on the wire contaminated with Staphylococcus aureus using the Total Plate Count (TPC) method with chlorhexidine 0,2% as a positive control and aquadest as negative control. The results of statistical test using One Way Anova showed there was a significant difference in the mean number of Staphylococcus aureus in all treatments, ant-nest plant mouthwash in concentration 0,8%, 1,6%, 3,2%, chlorhexidine 0,2%, and aquadest (p = 0,0001 < 0,05). This research concludes that ant-nest plant (Myrmecodia pendans) mouthwash in concentration 3,2% and chlorhexidine 0,2% have almost the same antibacterial effectiveness against Staphylococcus aureus.