Evaluasi Oral Wound Dressing Film yang Mengandung Kitosan terhadap Jumlah Neutrofil Pasca Insisi pada Gingiva Tikus Wistar (Rattus norvegicus)

Abstract

Gingivectomy is a periodontal surgical procedure that involves incising the gingiva, resulting in an open wound on the gingival surface. This procedure leaves the wound vulnerable to contamination, which can hinder the healing process and give rise to complications. To address this issue, the use of a periodontal dressing is necessary to minimize bacterial contamination and expedite wound healing. Chitosan, known for its antibacterial and anti-inflammatory properties, has shown promise in accelerating wound healing. The objective of this study is to investigate the impact of an oral wound dressing film containing chitosan on the increase in neutrophil count during the post-incision wound healing process in Wistar rats (Rattus norvegicus). A total of 45 Wistar rats will be divided into three groups: a treatment group receiving chitosan oral wound dressing film, a positive control group receiving Ora-Aid, and a negative control group receiving a placebo. The rats will be euthanized on days 1, 3, and 7 after incision to evaluate clinical parameters using Lanry's healing index, as well as histological examination to assess the neutrophil count. The findings of this study demonstrate that the application of oral wound dressing film containing chitosan can expedite the post-incision wound healing process in the gingiva of Wistar rats (Rattus norvegicus) by increasing the number of neutrophils.