Uji Aktivitas Antibakteri Ekstrak Daun Kecombrang (Etlingera elatior (Jack) R.M.Sm) terhadap Bakteri Enterococcus faecalis Berdasarkan KHM dan KBM

Abstract

Enterococcus faecalis is a Gram-positive anaerobic bacterium commonly found in cases of failure after root canal treatment, with at least 20 out of 30 cases showing its presence after root canal treatment. Torch ginger leaves have been reported to contain bioactive compounds that can inhibit bacteria. This study is an experimental laboratory research with a post-test only with controlled group design. The research samples consisted of Enterococcus faecalis ATCC® 29212TM and 70% ethanol extract of torch ginger leaves (Etlingera elatior) with 8 test concentrations and 2 control groups, each with 3 repetitions. The determination of minimum inhibitory concentration (MIC) in this study used the Kirby-Bauer disc diffusion method, and to determine the minimum bactericidal concentration (MBC), the streaking method from MIC testing was used. Data analysis for determining MIC values in this study used the parametric One-Way ANOVA test (p<0.05) followed by the Post Hoc Test (LSD), while the determination of MBC values used the Kruskal-Wallis test (p<0.05) followed by the Mann-Whitney test. The results of this study show that there is antibacterial activity of torch ginger leaf extract (Etlingera elatior) against Enterococcus faecalis bacteria. Torch ginger leaf extract is able to inhibit the growth of Enterococcus faecalis bacteria starting from concentrations of 3.125% to 80%, with an average inhibition zone of 6.90±0.46 mm and bactericidal efficacy of 29.49% to 14.93±0.87 mm with a bactericidal efficacy of 99.93%. This study concludes that the concentration of 3.125% is determined as the MIC value and falls into the moderate category. The MBC value of the 8 test concentration groups is at concentrations of 65%, 75%, and 80% because it is able to reduce the number of initial bacterial colonies by 98%-99%. The effective concentration of torch ginger leaf extract (Etlingera elatior) which has inhibitory and bactericidal efficacy and thus has the potential to become an irrigation material for root canals is the concentration of 65%.