Isolasi dan Identifikasi Golongan Senyawa Flavonoid dari Kulit Batang Sambung Nyawa (Gynura Procumbens (Lour.)Merr.)

Abstract

Isolation and identification of the flavonoid compound from the stem bark of sambung nyawa (Gynura procumbens (Lour.) Merr.) had been done by maceration extraction technique with methanol solvent. The methanol extract is concentrated, then the concentrated methanol extract is dissolved in ethyl acetate, concentrated and evaporated. The concentrated extract of ethyl acetate was dissolved with methanol and partition extracted with n-hexane. The methanol layer is then evaporated until all the methanol has evaporated. The concentrated methanol extract was then analyzed by TLC, then separated by column chromatography with silica gel as the stationary phase and chloroform:methanol (90:10, 80:20, 70:30, 60:40)v /v as the mobile phase.The compund obtained was purified by preparative thin layer chromatography with chloroform : ethyl acetate (80:20) v /v. eluent and yellowy green amorphous solid was obtained as much as 18 mg with Rf=0,62 using chloroform : ethyl acetate (80:20) v /v. The UV-Visible spectrum show a wavelength (λmax) 324 nm. The FT-IR spectrum shows the presence of OH stretch, C-H sp3 stretch, C=O stretch, C=C aromatis stretch, C-O stretch, C-H sp2 bend out-of-plane respectively according to wave 3431,36; 2924,09; 1656,85; 1543,05; 1103,28 dan 800,46 (cm-1 ). The 1HNMR shows the presence of protons H-2, H-2’, H-6’, and H-8. Based on the interpretation of spectroscopic data, it shows that the isolated compound is a flavonoid compound from the isoflavone group.