Pengaruh Efektivitas Kayu Manis (Cinnamomum Burmannii) dan Bunga Cengkeh (Syzygium Aromaticum) sebagai Antiseptik terhadap Pertumbuhan Bakteri Escherichia Coli

Abstract

Unclean hands can transfer Escherichia coli from the body, feces, or other sources to food which when consumed can cause diarrhea. The use of natural ingredients as antiseptics is one solution to maintain hand hygiene. The purpose of this study was to determine the influence of the effectiveness of cinnamon (cinnamomum burmannii) and clove (syzygium aromaticum) as antiseptics on the growth of escherichia coli bacteria. This study is a quantitative study with a true experimental method with a post-test only control design. Testing the inhibition of Escherichia coli in antiseptic extracts of cinnamon and clove using the Kirby-Bauer test with 3 repetitions. The extracts used were obtained from the maceration process and made into several concentrations, namely 20%, 40%, 60%, 80%, and 100%. The positive controls used were Chloramphenicol Palmitate antibiotics and 70% alcohol antiseptic. The negative control in this study was aquadest. The measurement results after 24 hours showed that cinnamon and clove extracts at concentrations of 20%, 40%, 60%, 80%, and 100% respectively effectively inhibited the growth of Escherichia coli because there were clear zones of 10,067 mm, 10,867 mm, 11,267 mm, 12,833 mm, and 13,4 mm. Data analysis using the Kruskal-wallis test showed p-value was 0,026 (p < 0,05). This means there is a difference in the average diameter of the bacterial inhibition zone. Through the Post Hoc Multiple Comparison test, it was found that 100% concentration with 70% alcohol antiseptic had a significant difference because it had a p-value = 0,036 (p < 0,05). However, concentrations of 20% to 80% showed no significant difference with 70% alcohol antiseptic, because the p-value showed p > 0,05. Therefore, cinnamon and clove antiseptic from 20% to 100% concentration can be a substitute for 70% antiseptic alcohol. Future research can test cinnamon and clove antiseptics on other bacteria