Karakterisasi Film dari Pati Singkong (Manihot Esculenta) sebagai Antibakteri Pseudomonas Aeruginosa pada Luka Sayat

Abstract

Films are thin sheets used to close and protect wounds. Films are often made from materials such as starch and chitosan as an antibacterial agent for Pseudomonas aeruginosa. Film making in the form of plaster is by mixing cassava starch with variations of 5; 6; 7 and 8 grams and distilled water with variations of 78; 77; 76 and 75 mL, chitosan with a fixed composition of 2%, added with 2 mL of glycerol, stirred until homogeneous, printed on an acrylic plate, dried in the oven for ± 2 days. The best results were obtained 8 grams of cassava starch, 75 mL of distilled water, 50 mL of 2% chitosan, and 2 mL of glycerol. From the research results on this variation gives the best results with characteristic values such as thickness, tensile strength, and SEM which are 1.21 MPa, 0.3 mm, has a surface structure that is increasingly organized and compatible. The best film variation was tested for antibacterial against Pseudomonas aeruginosa by disc diffusion method. The film with a variation of 8 grams of cassava starch produced an inhibition zone of 13,6 mm which was categorized as a strong inhibition zone. The film in the form of plaster with the best inhibition zone was tested preclinically on male white rats. Observation of the incision wound given to white rats, can be healed within 6 days.