Pengaruh Lama Waktu Aplikasi Pasta Hidroksiapatit Cangkang Telur 10% (Anas platyrhnchos) sebagai Bahan Remineralisasi Alami terhadap Kekerasan Email

Abstract

Tooth structure can undergo demineralization and remineralization. Demineralization of teeth can be addressed using Casein Phosphopeptide Amorphous Calcium Phosphate (CPP-ACP), which can trigger allergic reactions in individuals allergic to milk. Therefore, alternative materials such as hydroxyapatite from duck eggshells, whose mineral content closely resembles that of teeth, are needed. This study aims to investigate the duration of application of duck eggshell hydroxyapatite paste on enamel hardness. This laboratory experimental study employed a pre- and post-test with control group design using 24 extracted premolar teeth samples divided into 4 groups. After preparing the hydroxyapatite powder, FTIR analysis was conducted. Samples were immersed in soda drinks to induce demineralization effects, followed by surface enamel hardness testing. Each group received the respective test material: Group I received 10% duck eggshell hydroxyapatite paste applied for 15 minutes, Group II for 30 minutes, Group III for 60 minutes, and Group IV received CPP-ACP paste applied for 3 minutes. The procedures were conducted over 5 days, and surface hardness was tested using a Microvickers Hardness Tester. Data were analyzed using paired t-tests, one-way ANOVA, and Post Hoc tests. The paired t-test results indicated significant changes in hardness for Groups A (p=0.009), B (p=0.012), C (p=0.0001), and D (p=0.004). One-way ANOVA results showed significant differences in hardness among the four groups (p=0.017). Post Hoc analysis revealed no significant difference between Groups A and D (p=0.874), Groups B and C (p=0.360), but a significant difference between Groups C and D (p=0.007). In conclusion, the application of test materials in Groups A, B, C, and D influenced surface enamel hardness, with significant differences observed between Groups C and D.