| dc.description.abstract | Background: Immunomodulators are compounds that can stimulate or improve the
function of the immune system. Flavonoids such as hesperidin and naringenin
found in ethanol extract of sweet orange peel (Citrus sinensis) can act as
immunostimulants
Objective: To determine the immunomodulatory effects of ethanol extract of sweet
orange peel (Citrus sinensis) on phagocytosis activity and delayed-type
hypersensitivity response
Method: Testing was performed using the carbon clearance method, where mice
were orally treated for 7 days with CMC-Na 0.5%, Imboost® 32.5 mg/kg BB, and
ethanol extract of sweet orange peel at doses of 25, 50, and 100 mg/kg BW. On the
8th day, carbon suspension was intravenously administered, followed by blood
collection and measurement of absorbance using a UV-Vis spectrophotometer. After
12 hours, the mice were dissected to collect liver and spleen. In the delayed-type
hypersensitivity method, rats were orally treated for 14 days with CMC-Na 0.5%,
levamisole 25 mg/kg BW, and ethanol extract of sweet orange peel at doses of 17.5,
35, and 70 mg/kg BW. On the 4th and 14th days, rats were injected with
Staphylococcus aureus, and the volume of footpad swelling was measured
Results: The results showed that treatment with ethanol extract of sweet orange
peel at doses of 25, 50, and 100 mg/kg BW significantly increased carbon clearance
rate, phagocytosis index, and stimulation index in mice compared to the negative
control CMC-Na 0.5% (p < 0.05). Administration of ethanol extract of jeruk manis
peel at doses of 17.5, 35, and 70 mg/kg BW also resulted in significant footpad
swelling in rats compared to the negative control CMC-Na 0.5% (p < 0.05)
Conclusion: Ethanol extract of sweet orange peel has immunostimulant activity as
evidenced by increased phagocytic activity in male mice and delayed-type
hypersensitivity response in male rats. | en_US |
