Efektivitas Pemberian Ekstrak Metanol Daun Pegagan (Centella asiatica (L.) Urban) dengan Dosis 200 mg/kg BB Menggunakan Metode Microwave Assisted Extraction (MAE) Berdasarkan Pengujian Total Fenol terhadap Kejadian Displasia pada Tikus Wistar

Abstract

Dysplasia or also called precancerous lesions are lesions that experience changes in the epithelial tissue (stratified squamous epithelium) characterized by cellular atpia, loss of maturation and stratification which has the potential to become malignant. The compound 7,12-dimethylbenz (α) anthracene (DMBA) is one of the compounds in the PAH group that meets the minimum criteria for carcinogenic potential. The pegagan leaves (Centella asiatica (L.) Urban) is a natural ingredient that can be used as a chemopreventive agent because it contains many bioactive compounds in the form of alkaloids, flavonoids, glycosides, saponins, tannins, triterpenoids/steroids. As well as polyphenols which have antioxidant properties which have the potential to prevent cancer. This research aims to determine the effectivity of methanol extract of pegagan leaves (Centella asiatica (L.) Urban) with a dose of 200 mg/kg BW using the microwave assisted extraction (MAE) method based on total phenol testing for the incidence of dysplasia in Wistar rats. This research is a laboratory experimental study with a sample of 20 Wistar rats (Rattus norvegicus) and divided into 2 groups. In group 1, samples were given methanol extract of pegagan leaves kola leaves at a dose of 200 mg/Kg BW and induced by DMBA three times per week, group 2 samples were only given DMBA three times per week. The degree of dysplasia in this study was measured using a scoring system WHO 2017. The results of measuring the degree of dysplasia were then analyzed using descriptive tests and continued with the Chi-square test. The results of this study show a p value of 0.301, so the p value is > 0.05, which means the The effectivity of methanol extract of pegagan leaves (Centella asiatica (L.) Urban) with a dose of 200 mg/kg BW using the microwave assisted extraction (MAE) method based on total phenol testing on the incidence of dysplasia in Wistar rats was less effective on the incidence of dysplasia because the difference with the control group was not significant.