Pengaruh Penambahan Gel Ekstrak Oregano (Origanum vulgare) pada Soft Denture Liner Silikon Swapolimerisasi terhadap Zona Hambat Candida Albicans Berdasarkan Waktu Pemakaian

Abstract

Auto-polymerized soft denture liner silicone is one of the most frequently used relining materials. However, long-term use of auto-polymerizing silicone SDL can cause bond failure between the soft liners and the denture, porosity dan changes in surface hardness, which will cause denture stomatitis. To overcome this, prevention is carried out by adding antifungal ingredients to the soft liners, such as oil extracted from oregano leaves (Origanum vulgare). The aim of this research was to determine the effect of adding Origanum vulgare extract gel to auto-polymerizing silicone soft dentures on the inhibition zone of Candida albicans based on 1, 7 and 14 days of used. The inhibition zone was carried out using the disc diffusion method. Research data analysis was tested using a univariant test to determine the mean value and standard deviation (SD) of the inhibition zone for each treatment group, a one-way ANOVA test to determine the effect of additions, and a repeated ANOVA test to determine the effect of usage time. The research results showed that the Minimum Inhibition Concentration (MIC) of oregano extract using the steam distillation method is 0,781%. The results of the univariant test showed that the mean and SD values of the inhibition zone in the group without the addition of antifungal agents with usage of 1, 7 and 14 days respectively were 0 mm; the nystatin gel addition group were 12.22 ± 0.17 mm, 10.77 ± 0.23 mm, and 8.99 ± 0.13 mm; and the group adding oregano extract gel were 19.72 ± 0.59 mm, 19.09 ± 0.43 mm, and 18.62 ± 0.41 mm. The results of the one-way ANOVA test showed that there was an effect of adding oregano extract gel to auto-polymerized silicone SDL on the inhibition zone of Candida albicans days based on usage of 1, 7, and 14 with a value of p<0.001 (p<0.05). Repeated ANOVA test results showed that there was an effect of usage of 1, 7, and 14 days on the Candida albicans inhibition zone on auto-polymerized silicone SDL with the addition of nystatin gel and with the addition of oregano extract gel with each group's p value <0.001 (p<0.05). From the results of this study, it can be concluded that the addition of Origanum vulgare extract gel with MIC 0,781% to auto-polymerizing silicone SDL are more effective in inhibiting the growth of Candida albicans compared to the addition of nystatin gel, although the inhibition zone will decrease over time of use.